Characterization of oxidative enzyme changes in buffalograsses challenged by Blissus occiduus.
نویسندگان
چکیده
This research investigated the role of oxidative enzymes in the defense response of buffalograss, Buchloë dactyloides (Nuttall) Engelmann, to Blissus occiduus Barber. Changes in catalase and peroxidase activity were observed in both resistant and susceptible buffalograsses in response to chinch bug feeding. Susceptible plants were shown to have a lower level of catalase activity compared with their respective control plants. By contrast, catalase activities of resistant plants were similar between infested and control buffalograsses throughout the study. Resistant plants had higher levels of peroxidase activity compared with their control plants, whereas peroxidase activities for control and infested susceptible plants remained at similar levels or were slightly lower for infested plants. These findings suggest that chinch bug feeding leads to a loss in catalase activity in susceptible buffalograsses. In contrast, resistant buffalograsses may be able to tolerate chinch bug feeding by increasing their peroxidase activity. Polyphenol oxidase activities were similar between control and infested plants for the buffalograsses evaluated. Among the enzymes examined, no differences in isozyme profiles for peroxidase and polyphenol oxidase were detected between control and infested 378, NE91-118, Cody, and Tatanka plants. Gels stained for catalase identified differences in the isozyme profiles of infested and uninfested 378 plants; however, infested and control NE91-118, Tatanka, and Cody plants has similar isozyme profiles. No differences in protein profiles were observed between chinch buginfested 378, NE91-118, Cody, and Tatanka plants and their respective uninfested controls.
منابع مشابه
Characterization of Oxidative Enzyme Changes in Buffalograsses Challenged by <i>Blissus occiduus</i>
This research investigated the role of oxidative enzymes in the defense response of buffalograss,Buchloë dactyloides (Nuttall) Engelmann, toBlissus occiduusBarber. Changes in catalase and peroxidase activity were observed in both resistant and susceptible buffalograsses in response to chinch bug feeding. Susceptible plants were shown to have a lower level of catalase activity compared with thei...
متن کاملEvaluation of cool- and warm-season grasses for resistance to multiple chinch bug (Hemiptera: Blissidae) species.
Chinch bugs are common pests of many agronomic and horticulturally important crops and turfgrasses. The extensive overlap of plant hosts and geographic distribution of Blissus leucopterus leucopterus (Say), Blissus leucopterus hirtus Montandon, Blissus insularis Barber, and Blissus occiduus Barber underscores the importance of identifying resistant germplasm. Cool- and warm-season turfgrasses a...
متن کاملPhysiological responses of resistant and susceptible buffalograsses to Blissus occiduus (Hemiptera: Blissidae) feeding.
The impact of Blissus occiduus Barber feeding on resistant ('Prestige') and susceptible ('378') buffalograsses, Buchloë dactyloides (Nuttall) Engelmann, was evaluated through measurement of carbon exchange rate, light and carbon assimilation (A-C(i)) curves, chlorophyll a fluorescence, and nonstructural carbohydrates. No significant differences in carbon exchange rates were observed between inf...
متن کاملPhysiological Responses of Resistant and Susceptible Buffalograsses to <i>Blissus Occiduus</i> (Hemiptera: Blissidae) Feeding
The impact of Blissus occiduus Barber feeding on resistant (ÔPrestigeÕ) and susceptible (Ô378Õ) buffalograsses,Buchloë dactyloides (Nuttall) Engelmann, was evaluated throughmeasurement of carbon exchange rate, light and carbon assimilation (A-Ci) curves, chlorophyll a ßuorescence, and nonstructural carbohydrates. No signiÞcant differences in carbon exchange rates were observed between infested ...
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ورودعنوان ژورنال:
- Journal of economic entomology
دوره 97 3 شماره
صفحات -
تاریخ انتشار 2004